2011•Xiandai shengwu yixue jinzhanRequires access
Effects of magnesium isoglycyrrhizinate on hepatic TGF-β 1 and Smad protein expression in rats with experimental hepatic fibrosis
Peng Zhong-tian
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Abstract
Objective:To discuss the probable effects of Magnesium Isoglycyrrhizinate on rats of experimental hepatic fibrosis and its potential molecular mechanisms.Methods:Healthy male SD rats were randomly divided into different groups:normal control,model of hepatic fibrosis,INF-γ-treated and Magnesium Isoglycyrrhizinate-treated groups.Hepatic fibrosis in healthy male SD rats was induced by intraperitoneal injection of Tetrachloride.The serum concentration of Hyaluronic acid(HA),Laminin(LN),Procolagen Type III(PCIII) and Collagen Type IV(C-IV) were assayed with Radioimmunoassay.The mRNA levels of TGFβ1,Smad 3,and Smad 7 in rat's liver tissue were detected with RT-PCR.Results:Compared with model group,the level of serum HA,LN,PC-III,C-IV in Magnesium Isoglycyrrhizinate groups were significantly decreased(P0.05),the pathological fibrosis scores were also decreased obviously(P0.05),and the expression of TGF-β1,Smad3 mRNA in rat's liver tissue were decreased significantly(P0.05),but Smad7 mRNA was increased.Conclusion:The mechanisms of Magnesium Isoglycyrrhizinate on protecting against tetrachloride——induced liver fibrosis in SD rats may be reduced the expression of TGF-β1,Smad3 mRNA,and increase the expression of Smad7 mRNA at the same time.