2011•Zhongguo yiyuan ganranxue zazhiRequires access

Detection of mycoplasma pneumoniae among children with respiratory tract infections in Guizhou

Yue Wu

Abstract

OBJECTIVE To evaluate the methods for detection and diagnosis of mycoplasma infections in children with pneumonia.METHODS A total of 268 samples were collected from the patients with MP,and mp-antibody was detected by using passive agglutination(PA) method,MP-DNA loads was examined by real-time PCR.RESULTS Of 268 patients with pneumoniae,68 children were confirmed to be positive of Mycoplasma pneumoniae.The positive rate of detection was significantly higher with PCR than with antibody(P0.05).There was no significant difference between the specificity of the two methods(P0.05).CONCLUSION PCR method and PA are highly applicable in clinical laboratories for the preliminary detection and diagnosis of mycoplasma infections.

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Key concepts: Mycoplasma pneumoniae, Mycoplasma, Pneumonia, Mycoplasma pneumonia, Respiratory tract infections, Respiratory tract, Medicine, Microbiology