Expression of cDNA fragment of drug resistance-related gene of human lung adenocarcinoma in gastric cancer

Objective To study the expression of the drug resistance related gene fragment found in our previous studies by the suppression subtractive hybridization(SSH) in the gastric cancer and normal tissues. Methods The expression was detected by in situ hybridization histochemistry(ISHH) using labeled digoxigenin probes in the gastric cancer and normal tissues. Results Expression of the gene fragment was found in most of the gastric adenocarcinoma tissues detected, but not in other gastric adenocarcinoma, normal tissues and signet ring cell carcinoma tissues. There was significant difference between gastric adenocarcinoma tissues and normal tissues ( P 0.01), and it was the same case with gastric adenocarcinoma tissues and signet ring cell carcinoma tissues( P 0.01). Conclusion The fragment is expressed in most of the adenocarcinoma tissues. It may be an endogenetic gene fragment.