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Detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine with RT-PCR

Qingchun Shen

Open publisher page 7 citations

Abstract

In this study,RT-PCR was established for the detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine.Out of 23 samples detected with the established RT-PCR,5 were contaminated with BVDV.The sequences were analyzed by DNAStar and compared with the reference strains.The homology of the target BVDV gene sequence was 83.2%～83.5% with BVDV Oregon C24V(AF091605) and 86.4%～86.7% with NADL(M31182) respectively.

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What this paper is about

In this study,RT-PCR was established for the detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine.Out of 23 samples detected with the established RT-PCR,5 were contaminated with BVDV.The sequences were analyzed by DNAStar and compared with the reference strains.The homology of the target BVDV gene sequence was 83.2%～83.5% with BVDV Oregon C24V(AF091605) and 86.4%～86.7% with NADL(M31182) respectively.

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OpenAlex reports 7 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

In this study,RT-PCR was established for the detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine.Out of 23 samples detected with the established RT-PCR,5 were contaminated with BVDV.The sequences were analyzed by DNAStar and compared with the reference strains.The homology of the target BVDV gene sequence was 83.2%～83.5% with BVDV Oregon C24V(AF091605) and 86.4%～86.7% with NADL(M31182) respectively.

Key concepts: Virology, Classical swine fever, Virus, Biology, Pestivirus, Diarrhea, Real-time polymerase chain reaction, Polymerase chain reaction

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