Establishment and Initially Application of Double RT-PCR Detection Method for Both Classical Swine Fever Virus and Bovine Viral Diarrhea Virus

According to the complete genome sequences of classical swine fever virus(CSFV) and bovine viral diarrhea virus(BVDV) in GenBank,two pairs of primers were designed and synthesized.A double RT-PCR was developed based on these two pairs of primers which amplified the CSFV virus-specific segment with 938 bp and the BVDV virus-specific segment with 650 bp in sizes after the conditions of PCR were optimized.Approved this method of 11 batches of bovine testicular cells,7 batches of fetal bovine serum,60 batches of swine fever vaccine,10 full-blood and 10 tissue samples for testing.The experiment showed that the developed method has good specificity and sensitivity of cell vaccine to prevent the pollution of BVDV and the diagnosis of CSFV and BVDV provides an effective method.