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Primarily Analyzing on Expressed Sequence Tags from Chicken Hypothalamus cDNA Library

Zhihui Zhao

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Abstract

In order to monitor quality of the chicken hypothalamus cDNA library constructed and whether it could be used to construct the expression profile of hypothalamus,we sequenced and primarily analyzed 12 ESTs from randomly selected clones.By BLASTn and FASTA analysis in GenBank,1 EST could find homology in chicken,4 had matching sequences in turkey or other species,1 had homologous EST sequence in EST database,6 were function unknown gene.All 12 ESTs sequence were loaded into GenBank.

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What this paper is about

In order to monitor quality of the chicken hypothalamus cDNA library constructed and whether it could be used to construct the expression profile of hypothalamus,we sequenced and primarily analyzed 12 ESTs from randomly selected clones.By BLASTn and FASTA analysis in GenBank,1 EST could find homology in chicken,4 had matching sequences in turkey or other species,1 had homologous EST sequence in EST database,6 were function unknown gene.All 12 ESTs sequence were loaded into GenBank.

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Available abstract

In order to monitor quality of the chicken hypothalamus cDNA library constructed and whether it could be used to construct the expression profile of hypothalamus,we sequenced and primarily analyzed 12 ESTs from randomly selected clones.By BLASTn and FASTA analysis in GenBank,1 EST could find homology in chicken,4 had matching sequences in turkey or other species,1 had homologous EST sequence in EST database,6 were function unknown gene.All 12 ESTs sequence were loaded into GenBank.

Key concepts: GenBank, Biology, Expressed sequence tag, cDNA library, Complementary DNA, Homology (biology), Genetics, Gene

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