2002•Zhongguo nongye KexueRequires access

Analysis of Expressed Sequence Tags from Porcine Liver Organ

Ning Li

Abstract

In order to study the expression of functional gene and its effect on metabolic control and other physiological functions in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species, while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size and the results showed that 19 of them were function known genes, 11 had no open reading frame ( ORF ) and 15 had ORF but the function were not elucidated yet.

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Key concepts: GenBank, Expressed sequence tag, Open reading frame, Biology, Complementary DNA, Gene, cDNA library, Genetics