Establishment of mice model with learning and memory deficits induced by cerebral ischemia/reperfusion

Objective:To establish mice model with learning and memory deficit induced by cerebral ischemia/reperfusion.Methods:The mice model induced by cerebral ischemia/reperfusion were made by bilateral occlusion of both common carotid arteries and ischemic hypotension/reperfusion.Locomotive activity,step-down test and water maze were used to reflect behavior changes,as well as cell morphologic changes were evaluated,respectively.And total anti-oxidant capacity and activities of SOD(superoxide dismutase)and MDA(malondialdehyde)were also measured.Results:The neuronal damage in model mice was significant,compared with sham group.And locomotive activity of model mice markedly decreased.The escape latency and time of electric shock of model mice significantly increased in step-down test;and 24 hours later the number of errors significantly increased,step-down latency and time of remaining on platform markedly decreased.In maze test,the spatial learning and memory ability of model mice significantly decreased with characteristics of increase in the number of errors and latency at D3,D4 and D5,and the total anti-oxidant capacity and activities of SOD were decreased and MDA level increased in model group compared with sham group,respectively.Conclusion:This model of mice cerebral ischemia/reperfusion is useful for studying learning and memory deficit.