Etiological Analysis of Hand Foot and Mouth Disease in Jiangxi

Objective To investigate the etiological characteristics of children with hand foot and mouth disease(HFMD) in Nanchang to provide the basis for clinical diagnosis Methods TaqMan Real-Time RT-PCR technique was used for genetic detection of enterovirus 71(EV71),coxsackievirus A16(CA16) and universal enterovirus(EV) in 768 throat swab samples from patients with suspected HFMD. Results Among the 768 samples,489(63.67%) were EV PCR-positive,233(30.34%) were EV71 PCR-positive,and 103(13.41%) were CA16 PCR-positive.EV71 positive rate in inpatients was 36.67%,which was obviously higher than that(21.88%)in outpatients.Therefore,EV71 was the main pathogen causing severe symptoms.Conclusion The major causative agents of HFMD include EV71(30.34%) and CA16(13.41%) and EV71 PCR-positive rate in inpatients is obviously higher than that in outpatients,suggesting that EV71 is the main pathogen causing severe HFMD.