Construction and identification of CD147 shRNA expression vector

Objective To construct the expressing vector of short hairpin RNA ( shRNA) sections targeting human CD147 gene. Methods Two pairs of specific CD147 shRNA oligoes were designed and synthesized according to CD147 cDNA sequence and the principle of shRNA designing. The complement form was cloned into plasmid vector pGenesil-1.1, identified by digestion and tested for the sequence. Results The recombinant pGenesil-1.1 plasmid was cloned and the CD147 cDNA sequence was obtained. The digestion of plasmid vectors identified that DNA template primer was successfully inserted into the plasmid and the sequence was in conformity with the designed result. Conclusion Successful construction of human CD147 shRNA expressing vectors provides a favorable foundation for further study on the function of CD147.