Extraction of alginates by enzymatic hydrolysis

Alginates was extracted from laminaria by hydrolysis of cellulase,pectinase and protease and its extraction processes has been optimized.The results indicated that the optimal hydrolysis conditions were:2% cellulase (at 55℃ and pH 4.5 for 20 min),1% pectinase (at 60℃ and pH 4.5 for 1.5h) and 1% protease (at 80℃and pH 8.0 for 3h).And then all the enzymes were inactived by boiling solution for 10 min.1 mol/L CaCl2 was finally added to the hydrolysis solution with the volume ratio of 1:4 (the hydrolysis solution:CaCl2 solution).In the end,alginates was obtained by bleaching,acidification,drying and alkalization.