2013Northern HorticultureRequires access

Study on the Optimum of Extraction Techniques for Pectin from Banana Peel

Zhenlin Chen

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Abstract

Taking banana as material,the pectin was extracted from banana peel by acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis respectively and the effect was compared.The results showed that pectin yields of acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis were 16.57%,17.47%,20.92%in turn.Therefore,enzyme hydrolysis was superior to acid hydrolysis,and acid-enzyme hydrolysis was much better than enzyme hydrolysis and acid hydrolysis according to pectin yields.The optimal conditions of acid-enzyme hydrolysis were as follows : during the acid hydrolysis,ratio of solid to liquid 1∶20,pH 1.0,extraction temperature 70℃,extraction time 90 min;during the enzyme hydrolysis,cellulase concentration 0.25%,pH 5.0,enzyme hydrolysis temperature 50℃and enzyme hydrolysis time 25 min.

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What this paper is about

Taking banana as material,the pectin was extracted from banana peel by acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis respectively and the effect was compared.The results showed that pectin yields of acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis were 16.57%,17.47%,20.92%in turn.Therefore,enzyme hydrolysis was superior to acid hydrolysis,and acid-enzyme hydrolysis was much better than enzyme hydrolysis and acid hydrolysis according to pectin yields.The optimal conditions of acid-enzyme hydrolysis were as follows : during the acid hydrolysis,ratio of solid to liquid 1∶20,pH 1.0,extraction temperature 70℃,extraction time 90 min;during the enzyme hydrolysis,cellulase concentration 0.25%,pH 5.0,enzyme hydrolysis temperature 50℃and enzyme hydrolysis time 25 min.

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Available abstract

Taking banana as material,the pectin was extracted from banana peel by acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis respectively and the effect was compared.The results showed that pectin yields of acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis were 16.57%,17.47%,20.92%in turn.Therefore,enzyme hydrolysis was superior to acid hydrolysis,and acid-enzyme hydrolysis was much better than enzyme hydrolysis and acid hydrolysis according to pectin yields.The optimal conditions of acid-enzyme hydrolysis were as follows : during the acid hydrolysis,ratio of solid to liquid 1∶20,pH 1.0,extraction temperature 70℃,extraction time 90 min;during the enzyme hydrolysis,cellulase concentration 0.25%,pH 5.0,enzyme hydrolysis temperature 50℃and enzyme hydrolysis time 25 min.

Key concepts: Hydrolysis, Chemistry, Cellulase, Enzymatic hydrolysis, Pectin, Extraction (chemistry), Enzyme, Chromatography

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