Study on the Optimum of Extraction Techniques for Pectin from Banana Peel
Zhenlin Chen
Abstract
Zhenlin Chen
Abstract
Taking banana as material,the pectin was extracted from banana peel by acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis respectively and the effect was compared.The results showed that pectin yields of acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis were 16.57%,17.47%,20.92%in turn.Therefore,enzyme hydrolysis was superior to acid hydrolysis,and acid-enzyme hydrolysis was much better than enzyme hydrolysis and acid hydrolysis according to pectin yields.The optimal conditions of acid-enzyme hydrolysis were as follows : during the acid hydrolysis,ratio of solid to liquid 1∶20,pH 1.0,extraction temperature 70℃,extraction time 90 min;during the enzyme hydrolysis,cellulase concentration 0.25%,pH 5.0,enzyme hydrolysis temperature 50℃and enzyme hydrolysis time 25 min.
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Taking banana as material,the pectin was extracted from banana peel by acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis respectively and the effect was compared.The results showed that pectin yields of acid hydrolysis,enzyme hydrolysis and acid-enzyme hydrolysis were 16.57%,17.47%,20.92%in turn.Therefore,enzyme hydrolysis was superior to acid hydrolysis,and acid-enzyme hydrolysis was much better than enzyme hydrolysis and acid hydrolysis according to pectin yields.The optimal conditions of acid-enzyme hydrolysis were as follows : during the acid hydrolysis,ratio of solid to liquid 1∶20,pH 1.0,extraction temperature 70℃,extraction time 90 min;during the enzyme hydrolysis,cellulase concentration 0.25%,pH 5.0,enzyme hydrolysis temperature 50℃and enzyme hydrolysis time 25 min.
Key concepts: Hydrolysis, Chemistry, Cellulase, Enzymatic hydrolysis, Pectin, Extraction (chemistry), Enzyme, Chromatography