2004•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Influence of combined nimesulide and mitomycin on proliferation and apoptosis to human gastric cancer cell
Jianguo Cao
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Abstract
objective: This study was designed to evaluate the potential role of Nimesulide(NIM), a selective Cyclooxygenase-2 (COX-2) inhibitor, Combined with Mitomycin-c( MMC-c) on proliferation and apoptosis of gastric cancer cell lines SGC7901. Methods: MTT assay and clone formation rate were used to determine the influence of NIN Combined with MMC on proliferation of gastric cancer cell lines SGC7901. Apoptosis was determined by Acridine Orange staining and flow cytometry. MTT assay were used to determine the influence of Nimesulide on MMC-inhibited proliferation of gastric cancer cell lines SGC7901 and MGC803 cells. MMC-c-induced apoptosis was determined by Acridine Orange staining and flow cytometry. Result: MTT and clone formation rate indicate: combined NIM and MMC, the IC50 decreased 0.74μg/ml (P0.01),clone formation relative rate decreased 46.4% (P0.01). Combined NIM and MMC, the apoptosis rate were enhanced noticeably compared with MMC or NIM along (P0.01). Conclusion: selective COX-2 inhibitors NIM can enhance drug toxicity of MMC-c to human MMC-c-inhibited proliferation and MMC-c-induced apoptosis of gastric cancer cell lines SGC7901 cells.