Effect and mechanism of Allicin on the proliferation of cisplatin-resistant ovarian cancer cell line of SKOV-3/DDP

Objective To investigate the effect and mechanism of Allicin on the proliferation of cisplatin-resistant ovarian cancer cell line of SKOV-3/DDP.Methods The SKOV-3/DDP cells were divided into four groups randomly,the Allicin group was treated by 40 μg/mL Allicin,the cicisplatin group was intervened by 40 μg/mL cicisplatin,the conbine group was treated by 40 μg/mL Allicin and cicisplatin,the control group was treated by nutrient fluid.All the groups were cultured for 24 hours and 48 hours.The cellular growth inhibition rate was calculated by MTT.RT-PCR was used to detect the expression of Bax and Bcl-2 mRNA;Western blot was used to detect the protein level of Bax and Bcl-2.Results The growth inhibition rate of the Cisplatin group,allicin group,combination group at each time point(24 h,48 h) was significantly higher than that in the control group,which in the combination group was significantly higher than that in the cisplatin group and the allicin group,all P0.05.The Bax mRNA and protein levels of Cisplatin group,allicin group and combined group at each time point were significantly higher than those in the control group,which in the Allicin group were significantly lower than those in the control group(all P0.05);the expression of Bcl-2 protein in cisplatin group at 48 h was significantly higher than that in the control group(P0.05);the Bax mRNA and protein levels in the combined group at each time point were significantly higher than those in the control group,but the Bcl-2 mRNA and protein levels were significantly lower than those in the control group,all P0.05.Conclusions The SKOV-3/DDP apoptosis-inducing effects of allicin is superior to cisplatin,tcombination of the two drugs has a synergistic effect,its mechanism may be related to the upregulation of Bax expression and downregulation of Bcl-2 expression.