Effect of “Shidao Tongjie Decoction ” on proliferation and apoptosis of esophageal cancer cell lines TE-1

Objective To investigate the effect of Shidao Tongjie Decoction( SDTJ) on proliferation and apoptosis of esophageal cancer cell lines TE-1.Methods SD rats were established by serum containing SDTJ. The esophageal cancer cell lines TE-1 was used as the object. The appropriate concentration of drug serum was screened by MTT assay; Then serum,serum combined with cisplatin and cisplatin were applied to TE-1 cell lines. The growth of cancer cells were detected by MTT assay,and the cell cycle,apoptosis were detected by FCM. The expressions of apoptosis-related proteins( p-Akt,Akt,P53,Bcl-2,Bax,and Caspase3) were detected by Western blotting. Results ① The optimum concentration of the drug serum was 20%. ② MTT results showed that inhibition rates of serum group,cisplatin group and cisplatin plus serum group were fortified as the time goes by,and at 48h were 30. 9%,35. 4% and 50. 6%. There was a significant difference among the groups( P 0. 01). ③ Compared with the control group,the proportion in G1phase of the cisplatin group and cisplatin plus serum group were significantly higher( P 0. 01),and the proportion in G2phase of the serum group and cisplatin plus serum group were reduced( P 0. 05); The proportion in G1phase of the cisplatin plus serum group was higher than those of the cisplatin group and serum group,with significant difference between them( P 0.01). ④ FCM showed that there was a significant differences in apoptosis index of each intervention group at 12h and 24h( P 0.01); Compared with control group at different time-points,the apoptotic indexes of the cisplatin group,serum group and cisplatin plus serum group was markedly increased( P 0.01); the apoptotic indexes of the cisplatin group,cisplatin plus serum group were higher than that of the drug serum group( P 0. 01),the apoptotic index at different time-points of the cisplatin plus serum group higher than that of cisplatin group( P 0. 01). ⑤ Compared with the control group,the expressions of p-Akt and Bcl-2 in serum group,cisplatin group and cisplatin plus serum group were reduced( P 0. 01),the expressions of P53,Bax and Caspase3 were strengthened( P 0. 01). Conclusion Serum containing SDTJ might have the effect of inhibiting proliferation and promoting apoptosis on esophageal TE-1 cell line. And there shows the obvious synergies when it combined with cisplatin.