Protective effect of Shengmai injection to acute lung injury induced by paraquat in rats

【Objective】To study the protective effect of Shengmai injection against acute lung injury by paraquat (PQ).【Methods】50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups:blank group,negative control group,positive control group,Shengmai injection low-dose group (LDG),Shengmai injection highdose group (HDG).Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens.The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid.In the mean time,the level of MDA,superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui (BALF) were detected.And the activities of NK-кB and iNOS in lung tissue homogenate were measured.【Results】Compared with negative control group,lung congestion and lung edema of Shengmai injection low-dose group were mitigated.Lung wet weight/dry weight,rat of neutrophils,protein content were decreased (P 0.05,P 0.01).The MDA level decreased both in plasma and BALF (P 0.05),the SOD and GSH-Px activities were increased as well (P 0.01).The biological indexes and the contents of NK-кB and iNOS of lung injury were significantly decreased (P 0.05,P 0.01).【Conclusion】The lipid peroxidation damage was caused by acute poisoning of PQ,and NK-кB and iNOS could play an important role in lung injury of poisoned rats.Administration of Shengmai injection could improve the lipid peroxidation damage and ameliorate the lung injury and decrease the concentrations of NK-кB,iNOS caused by acute poisoning of PQ.