Comparison of transfection efficiency of Nucleofector~(TM) and liposome transfection techniques for transfection of recombinant plasmid into PA317 cells

Objective To compare the traditional transfection efficiency of liposome transfection technique and Nucleofector TM technique for the transfection of PLXSN CTLA4Ig TRES2 EGFP recombinant plasmid into PA317 cells. Methods Liposome transfection technique and Nucleofector TM technique were employed for the transfection of pLXSN CTLA4Ig TRES2 EGFP recombinant plasmid into PA317 cells, respectively. The expression of green fluorescent protein (EGFP) by PA317 cells and the positive cell rates were detected with inverted fluorescence microscope and flow cytometer for the comparison of the transfection efficiency by the two methods. Results Positive cells were not observed at 8 h after liposome transfection. Liposome transfection technique resulted in positive cell rates of (0 59±0 2)% and (4 39±2 43)% at 12 h and 24 h, respectively. However, Nucleofector TM technique revealed positive cells at 4 h, and resulted in positive cell rates of (12 27±3 38)% and (50 16±2 41)% at 12 h and 24 h, respectively. Conclusion Compared with the traditional liposome transfection technique, Nucleofector TM technique is a transfection technique with faster speed and higher efficiency.