Study on the polymorphism of shigella flexneri 2a by using AP-PCR and set1,set2 double-PCR

Objectives and Methods: APPCR and setl,set2 doublePCR were carried out in 71 strains of Shigella flexinert 2a,43 outbreak strains isolated from Inner Mongolia,28 strains isolated from Jiangmen. Results:All strains were divided into 2 different clones by using APPCR(perimer 12),four different clones by using APPCR(perimer 17) and setl,set2 doublePCR.Combining two methods,all strains were divided into 7 different clones,65 of 71 strains belong to a single clone,the others were not the same among themselves. Conclusions: APPCR and setl,set2 doublePCR were effective to analysis gene polymophism of Shigella.Majoring of 71strains,no matter isolated from different place and at different time,outbreak strains or sporadis,belong to a single clone,which caused Shigellosis in different places of our country at different times,must be given priority to research and control.