Amination of epoxy-containing agarose as a gene transfection vector

Agarose was degraded by oxidation,activated by chloroepoxypropane,and then aminated with ethylenediamine to introduce amino groups into agarose chain.Finally,the aminated agarose was combined to pEGFP-C1 plasmid.The infrared spectrophotometry analysis(IR),element analysis(EA),agarose gel electrophoresis,particle size and zeta potential analysis were carried out to characterize the as-prepared aminated agaorse and its application as a gene vector.The results of IR and EA showed that the agarose succeeded to be grafted with amino groups.The agarose gel electrophoresis showed that aminated agarose could effectively protect the pEGFP-C1 plasmid.The results provided a reasonable basis for the preparation of gene vector using aminated agarose.