Expression and clinical significance of P-gp,LRP,MRP and GST-π in non-small cell lung cancer tissues

Objective:To investigate the expression level and clinical significance of P-gp,LRP,MRP and GST-π in non-small cell lung cancer tissues. Methods: The expression of P-Glycoprotein(P-gp) lung cancer resistance protein(LRP),multidrug resistance protein(MRP) and glutathione S transferases(GST-π) were detected in 86 cases of lung cancer tissues from untreated patients by streptavidin-biotin-peroxidase(S-P) immunohistochemistry technique. Results: The positive rates of LRP and MRP in the adenocarcinoma and squamous carcinoma were 77.78%,51.22% and 80.00%,53.66% respectively.There were statistic difference in LRP and MRP expression in different tissues types(All P0.05).The positive rates of P-gp and GST-π in the adenocarcinoma and squamous carcinoma were 73.33%,60.98% and 80.00%,70.73% respectively.No significant difference was observed among the positive rates of P-gp and GST-π in different types of lung cancer(All P0.05).There were statistic difference in the expression positive rates except P-gp in all detected four resistance-related genes between the higher and lower differentiated degree(All P0.05).No significant difference was observed among the positive rates of four resistance-related genes in different TNM staging of lung cancer(All P0.05).Two kinds or three kinds or four kinds of resistance-related genes co-expression in the adenocarcinoma and squamous carcinoma tissues were 46.67% and 34.15%,31.11% and 24.39%,20.00% and 17.07% respectively.No significant difference was observed among the co-expression positive rates of various types in the adenocarcinoma and squamous carcinoma tissues(All P0.05).Conclusion: It has different degree overexpression of resistance-related genes in NSCLC tissues,multidrug resistence in adenocarcinoma was higher than that in squamous carcinoma,MDR of lung cancer is a process involved in polygene and multiple pathways.Combined detection of P-gp,LRP,MRP and GST-π may be useful for guiding the clinical chemotherapy of lung cancer and judgement the prognosis.