Expression and Localization of GFP-HPV16 E5 Fusion Protein in HeLa Cells

Objective To investigate the expression and localization of GFP - HPV16 E5 fusion proteins in HeLa cells.Methods HPV16 E5 gene fragment amplified by PCR was inserted into eukaryotic expression vector of pEGFP -C1 with the same restriction sites.Enzymes digestion and DNA sequencing were used to identify E5 gene fragment.The eukaryotic expression vector of pEGFP - C1/HPV16 E5 or pEGFP - C1 was transfected into HeLa cells,respectively.The expression and localization of GFP - HPV16 E5 or GFP was observed under fluorescent microscope.Results Fluorescence could be observed not only in the cytoplasma but also in the nuclei of the cells transfected by pEGFP - C1,and only in the cytoplasma of the cells transfected by pEGFP - C1/HPV16 E5.Conclusion Eukaryotic expression vector of pEGFP - C1/HPV16 E5 successfully expressed GFP - HPV16 E5 fusion proteins in HeLa cells and located in the cytoplasma.