Jianzhiliugao on Bleomycin Induced Pulmonary Fibrosis in Rats Model of PDGF-BB and the Expression of α-SMA Effects of Random Parallel Controlled Study

[Objective] To study the influence on PDGF-BB and α-SMA levels by Jianzhi-liquid on Bleomycin-induced Idiopathic interstitial pneumonia in rats models. [Methods]The use of random parallel control method, the number of 96 male Wistar rats of clean grade were randomly divided into 5 groups, A group(12 rats), B model group(28 rats), paste plus dexamethasone group C interstitial flow(28 rats), paste group D interstitial flow(28), dexamethasone group(E 28 rats). Anesthesia by intraperitoneal injection of rats with 10% chloral hydrate, amount of endoscopic tracheal intubation, intratracheal instillation of bleomycin 0.1mL normal saline(5mg/kg), drip immediately after the rats upright spin, so that the liquid medicine is evenly distributed in the lungs, pulmonary fibrosis model replication. A, group B with normal saline intraperitoneal injection of 0.3ml/d. Group C Jianzhiliugao gavage(24g dangshen, astragalus, Poria Atractylodes 18g, each 12g, Radix 20g, leech at the end of the research at 6g, the centipede powder at 2, 21g salvia, chuanxiong,peach kernel, earthworm each 15g, 12g of Radix Scutellariae, spreading hedyotis herb, coix seed 30g, dandelion 24g, Fritillaria thunbergii 15g 12g, zedoary, mountain arrowhead 21g, licorice 12g, forsythia, Herba Houttuyniae each 18g) the body weight of 10mL/kg rats(4.6g/10mL), intraperitoneal injection of dexamethasone 1mg/kg, 1 /d. Group D Jianzhiliugao gastric perfusion(4.6g/10mL) the body weight of 10mL/kg rats, 1 /d. E intraperitoneal injection of dexamethasone 1mg/kg, 1 /d. Continuous intervention for 4 weeks(28d). Experiment twenty-eighth days in group A, 3 rats were sacrificed by cervical dislocation, the other group were killed 7 rats exposed to rats, trachea, right lung perfusion of 10% Formaldehyde Solution, the pleural flattened the right lung in 10% Formaldehyde Solution fixed. Routine paraffin embedding, slicing(4 μm) for HE and immunohistochemical staining. Observation under light microscope, lung cells, lung tissue PDGF-BB, α-SMA.[Results]In B model group, alveolar structural damage, alveolar wall thickening, interstitial fibrosis and scar formation in lung, capillary lumen occlusion; C Jianzhiliugao plus dexamethasone group were mild to moderate pulmonary fibrosis, alveolar septal lesions confined, collagen deposition was lower than that of model group; D Jianzhiliugao group and the hormone group level pulmonary fibrosis was significantly reduced, changed slightly compared with normal group. E dexamethasone group did not change significantly in pulmonary fibrosis. The degree of pulmonary fibrosis from light to heavy as follows: A group, C group, D group, E group, B group. The value of PDGF-BB, compared with A group, B group each time increased significantly(P 0.05); compared with 7d group(P 0.01), D group and E group(P 0.05); compared with 14d group(P 0.01), D group and E group(P 0.05); comparison of 21d group(P 0.01), two two were(P 0.05); compared with 28d group(P 0.01), two two were(P 0.05).[Conclusion] Jianzhi-liquid could effectively low the expressions of PDGF-BB and α-SMA, and might be one of the mechanisms of Jianzhi-liquid treatment of pulmonary fibrosis.