Effect of Liuwei Dihuang Tang on expression of renal microRNA192 in diabetic rats

OBJECTIVE:To investigate the effects of Liuwei Dihuang Tang on renal function and expression of miR-192 and collagenⅠin diabetic rats.METHODS:The diabetic rat models were induced by intraperitoneal injection of streptozotocin(STZ) and randomly divided into model control group,captopril(Capoten) group and Liuwei Dihuang Tang group.Some normal other rats were used as normal control group.All rats were treated with corresponding drugs for 8 weeks.During and after the treatment,the general state,blood glucose levels,total urinary protein of the 24 h,serum creatinine and blood urea nitrogen contents,kidney weight and relative kidney weight were measured.The mRNA of miR-192,TGF-β_1 and collagenⅠin the kidney also were detected by quantitative real-time PCR.RESULTS:Diabetes mellitus and renal lesions occurred in the three model groups.The expression of miR-192,TGF-β_1 and collagenⅠmRNA of the kidney in diabetic rats increased obviously.Liuwei Dihuang Tang could improve the general state and renal function of the diabetic rats,decrease the blood glucose levels and the excretion rate of the 24 hour urine protein,reduce the expression of miR-192,TGF-β_1 and collagenⅠmRNA in kidney.CONCLUSION:Liuwei Dihuang Tang suppressed synthesis of miR-192 and collagenⅠ.Rehmanniae Decoction of Six Ingredients could ameliorate renal lesions in diabetic rats.