Research Progress of Lipase-Catalyzed Kinetic Resolutions of very Bulky Chiral Compounds

Lipase has been extensively used in kinetic resolution for producing optical pure compounds. but the enantio selectivity of a majority of lipase-catalyzed resolutions of racemize chiral compounds is not so ideal . At present, the research work of upgrading enantio selectivity of lipase is focused on reformation of lipase structure , reaction conditions optimization, improvement of asymmetic reaction process and enantio selective inhibitation. Some traditional and new rational approaches such as substrate design and limitation of size of substrate, high temperature biocatalysis and low temperature biocatalysis are introduced.