Construction and expression of HIV-1_(CN) Gag DNA vaccine

Aim To construct the eukaryotic expression vector for HIV 1 CN core protein Gag gene and express it in vitro . Methods The recombinant expression vector pVAXGAG was constructed by inserting Gag gene into DNA vaccine vector pVAX1. The recombinant vector was transfected into Hela cell via liposome and the expressed product was detected by indirect immnuofluo rescence (IF),Western blot and Dot ELISA. Results The indirect IF showed green fluorescence on the membrane of transfected cells. Western blot and Dot ELISA demonstrated that expressed Gag protein existed in the lysate of Hela cells transfected by recombinant plasmid. Conclusion The constructed DNA vaccine can be expressed in vitro , and the expressed protein can react specifically with anti HIV P24 mAb.