[Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1].

AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1. METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA. RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells. CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.