Effects of curcumin on proliferation and apoptosis of human ovarian cancer cell lineHO-8910 in vitro

Objective:To investigate the regulatory effects of curcumin on proliferation and apoptosis of human ovarian cancer cell line HO-8910 in vitro. Methods:Human ovarian cancer cell line HO-8910,cultured in vitro,was treated with 1.25-20 μg/ml curcumin for 6～24 h. Morphological changes of cells were observed with invert microscope, and growth inhibition rates of HO-8910 cells were measured with MTT method. The cell cycle distribution and apoptosis were detected by flow cytometic analysis. In addition, the expression of Fas,Fas-L,Bcl-2 and Bax in HO-8910 cell was observed by SABC immunohistochemistry. Results: Curcumin inhibited the proliferation of HO-8910 cells in a dose-and time-dependent manner. The cell growth was arrested at S and G2/M stage and aneuploid peak was observed by FCM. The expression of Fas,Fas-L,Bcl-2 was increased while the expression of Bax was both positive before and after treatment. Conclusion:The curcumin not only inhibits proliferation but also induces apoptosis of human ovarian cancer cell line HO-8910.