Establishment and preliminary application of multiplex PCR for rapid detection of Streptococcus suis serotype 2

Three pairs of primers were designed to amplify three genes, Streptococci genus specific gene (gdh), Streptococci suis species specific gene(16S rDNA)and Streptococci suis type 2 gene(cps2J). The PCR products were 725 bp, 523 bp and 387 bp, respectively. A multiplex PCR was established by optimizing the reaction conditions and used to detect 1 179 samples. The result showed that there were 667 strains of S. suis and 33 strains of S. suis type 2 in 1 105 strains of Streptococcus detected. A sensitive and specific multiplex PCR assay was established for the application in diagnosis and epidemiological survey of S. suis infection.