The anti-respiratory syncytial virus effect of an active compound(GC3-1-4) from Glycyrrhiza in vitro

Objective:To study the effect of anti-respiratory syncytial virus(RSV)on an active compound(GC3-1-4)from glycyrrhiza in vitro.Methods:The GC3-1-4 was isolated from glycyrrhiza,by means of hot water extraction,ethanol precipitation and column chromatography.The activity of GC3-1-4 against RSV was observed by cell culture while Ribavirin was used as a positive control.Results:The TC_(50) of GC3-1-4 was 0.45mg/mL,the EC_(50) of GC3-1-4 against RSV was 41.32μg/mL and TI was 10.89 The GC3-1-4 achieved a concentration-dependent inhibition of the replication of RSV in vitro.The GC3-1-4 showed and active effect against RSV replications in vitro when the GC3-1-4 was added at Oh,2h,4h,6h,8h,after virus infection.Chemical tests showed that GC3-1-4 was not glycyrrhizic acid or polysaccharide.Conclusion:The study suggested that GC3-1-4 was an obvious inhibitor to RSV in Hela cell culture.