Characterization of Ligninolytic Enzymes Production of a White-rot Fungus F-9

White-rot fungus F-9 was the fungus isolated newly.Used the PDA culture medium,the natural culture medium on liquid and the artificial culture medium on liquid carries on the raise on F-9 separately,and studied the production of enzyme.The study illustrated that its growth rate in PDA culture medium was quick,may reach 8 mm/d;and its growth rate and production of enzyme in natural culture medium on liquid quit to be also ideal,LiP was 341 U/L,MnP was 264 U/L;the peak activities of LiP and MnP produced by F-9 in the artificial culture medium on liquid appear later than the fungus in natural culture medium on liquid about 2~3 d,but the activities of the two enzyme were both greatly improved,LiP was 619 U/L,MnP was 443 U/L.Judging from the result,if optimize the train condition of F-9 further,it ought to get much better lignin degradation effect.