Influence of detergent in the measurement of haemagglutinin content of influenza vaccine by single radial immunodiffusion

Objective To research the influence of detergent in single radial immunodiffusion (SRID) test for the assay of haemagglutinin on different influenza vaccine intermedia products, and to determine the disintegration terms treating with detergent. Methods Examined the haemagglutinin content by single radial immunodiffusion assay after treating whole virus and subunit vaccine intermedia products with a variety of concentration and taking time of detergent, compared and analyzed on the measurement data. Results Size of the subunit vaccine diffusion zones increased with increasing concentrations of Zwittergent reaching 0.125%, and kept consistent with detergent concentrations higher than 0.125%. However, size of the whole virus diffusion zones increased until the detergent concentrations reach 1%. The result of haemagglutinin content did not varied by treating with 1% Zwittergent for 30, 60, and 120 min, there were no significant difference among the detergent results. Conclusion Treating with 1% Zwittergent for 30 min is proper for either whole virus or subunit vaccine intermedia products in single radial immunodiffusion for haemagglutinin assay, it is good for increasing the job efficiency, decreasing the experimental error and saving the experimentation costs.