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2011•Egyptian Journal of Agricultural ResearchOpen access

STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE

Eman Anwar Hassan, NABEL A. ABDEL WANIS, SUSAN K. TOLBA

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Abstract

Two experiments have been conducted to study incorporation of di-ethyl amino ethyl dextran (DEAE Dextran) during production of avian influenza (AI) inactivated vaccine. The first was planned to study addition of DEAE Dextran with different concentrations (25g, 50g and 100g/0.1ml) to the virus inoculum seed before egg inoculation. Obtained results revealed selection of 25g DEAE Dextran/0.1 ml that resulted in increasing haemagglutinating activity (HA) with 2 log and virus titre (EID50) with 3.2. In the second experiment, the same concentrations were added and tested separately during vaccine preparation with two different adjuvants. Serum of vaccinated chickens with the vaccine to which 100 g DEAE Dextran / 0.5 ml was added, developed satisfactory raise in antibody level in comparing to positive control groups which received the vaccine without any additives.

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Two experiments have been conducted to study incorporation of di-ethyl amino ethyl dextran (DEAE Dextran) during production of avian influenza (AI) inactivated vaccine. The first was planned to study addition of DEAE Dextran with different concentrations (25g, 50g and 100g/0.1ml) to the virus inoculum seed before egg inoculation. Obtained results revealed selection of 25g DEAE Dextran/0.1 ml that resulted in increasing haemagglutinating activity (HA) with 2 log and virus titre (EID50) with 3.2. In the second experiment, the same concentrations were added and tested separately during vaccine preparation with two different adjuvants. Serum of vaccinated chickens with the vaccine to which 100 g DEAE Dextran / 0.5 ml was added, developed satisfactory raise in antibody level in comparing to positive control groups which received the vaccine without any additives.

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Available abstract

Two experiments have been conducted to study incorporation of di-ethyl amino ethyl dextran (DEAE Dextran) during production of avian influenza (AI) inactivated vaccine. The first was planned to study addition of DEAE Dextran with different concentrations (25g, 50g and 100g/0.1ml) to the virus inoculum seed before egg inoculation. Obtained results revealed selection of 25g DEAE Dextran/0.1 ml that resulted in increasing haemagglutinating activity (HA) with 2 log and virus titre (EID50) with 3.2. In the second experiment, the same concentrations were added and tested separately during vaccine preparation with two different adjuvants. Serum of vaccinated chickens with the vaccine to which 100 g DEAE Dextran / 0.5 ml was added, developed satisfactory raise in antibody level in comparing to positive control groups which received the vaccine without any additives.

Key concepts: Dextran, Inactivated vaccine, Titer, Inoculation, Virus, Influenza vaccine, Chemistry, Virology

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