Research on Explants Sterilization Technique in Staphylea bumalda DC.Tissue Culture Process

[Objective] The aim was to seek appropriate explants sterilization method in Staphylea bumalda DC.tissue culture process.[Method] The comparative method was used to study the effect of alcohol,HgCl2,HgCl2+alcohol combination in different sterilization time on explants growth.[Result] The results showed that the appropriate explant sterilization technology was the shoot tips survival rate was 100% with 1% HgCl2+70% alcohol,processing time was 5 min+50 s respectively;The stem sections survival rate was 95% with 1% HgCl2,processing time was 15 min;The young leaves survival rate was 85% with 1% HgCl2,processing time was 12 min.[Conclusion] 1% HgCl2+70% alcohol,1% HgCl2 was effective sterilant of Staphylea bumalda DC.explants.