Effect of Tetramethypyrazine on the Proliferation and Apoptosis of Lung Cancer Cell Line of A549 and Its Mechanism

Objective:To investigate the inhibitory effect of tetramethypyrazine(TMP) on the proliferation and apoptosis of the lung adenocarcinoma A549 cell line and to explore its mechanism in the treatment of lung cancer.Methods:Different doses of TMP were added to the lung adenocarcinoma A549 cell line to demonstrate the biological function.MTT was performed to reveal the inhibitory effect on cell proliferation,and flow cytometry was used to determine the apoptosis and cell cycle of the lung adenocarcinoma A549 cell line after the tveatment of TMP.Bcl-2 and Bax were measured by immunocytochemistry.Results:There was a time-and dose-dependent inhibition of cell proliferation of A549 by TMP.TMP increased the rate of G0/G1 and G2/M of A549 cell cycle,down-regulate the expression of Bcl-2,and decrease the rate of Bcl-2/Bax so as to promote the apoptosis of A549.Conclusion:TMP can inhibit the proliferation of lung adenocarcinoma A549 cell line through changing cell cycle,down-regulating Bcl-2 expression,and decreasing the rate of Bcl-2/Bax.