Chiral Resolution of Fluoxetine and Carteolol byHigh Performance Capillary Electrophoresis

A method for separating enantiomers of fluoxetine and carteolol is described by HPCE using β _ cyclodextrin(β _ CD), carboxylmethyl _ β _ cyclodextrin(CM _ β _ CD), hydroxypropyl _ β _ cyclodextrin(HP_ β _ CD) and dimethyl _ β _ cyclodextrin(DM _ β _ CD) as a chiral selector. The separations were performed on an uncoated capillary of 50 cm×75 μm. The effects of types and concentrations of CDs in buffer on chiral resolution were investigated. The effects of pH and types of the background electrolytes on chiral resolution were also examined and separation conditions were optimized. It was observed that a good separation of carteolol or fluoxetine can be achieved by using approx 4 mmol/L of CM _ β _ CD, which not only saves analysis cost, but also simplifies the procedure. The mechanism of the chiral resolution was discussed briefly.