Comparative study on extraction methods of Dictyophora indusiata polysaccharide

The polysaccharide from Dictyophora indusiata was extracted by ultrasonic combined with multienzymatic method and the process was opitimized using orthogonal experimental design,compared with hot water method,ultrasonic wave method,cellulase method,pectinase method,papain method and multienzymatic method.The results showed that the optimal conditions of ultrasonic combined with multi-enzymatic extraction were material-water ratio 1∶50,enzymolysis time 60min,enzymolysis pH6,ultrasonic time 40min.The extraction ratio of Dictyophora indusiata polysaccharide was 16.35%,but it was 9.77% in hot water process,6.64% in ultrasonic process,8.84% in cellulase process,10.06% in pectinase process,10.35% in papain process and 11.27% in multi-enzymatic process,respectively,which were lower than that of ultrasonic combined with multi-enzymatic extraction.Therefore the extraction ratio of Dictyophora indusiata polysaccharide using ultrasonic combined with multi-enzymatic method was the highest,and it’s time of extraction was significantly shorter than that of hot water method.The ultrasonic combined with multi-enzymatic extraction was the best process among the 7 methods.