Development of a Directed Vat Set Starter of Lactic Acid Bacteria

A Directed Vat Set(DVS) of lactic acid bacteria(LAB) was developed for the fermentation and production of fermented soft drink and animal feed.Lactobacillus acidophilus LH1F was used as test strain.The cultural media for the L.acidophilus LH1F growth and the ingredients of anti-freeze protectants for lyophilization of LAB cells were optimized by orthogonal tests.The highest cell concentration of 4.25×1010 CFU/mL was found using tomato juice enrichment medium.The optimized anti-freeze protectants recipe contained skimmed milk powder 2.5%,glycerol 1%,glucose 2.5%,sucrose 1% and Vitamine C 2.5%.After 6 hours lyophilization,the number of viable LAB cells of DVS was about 1.27×1012 CFU/g.The cell number still maintained 3×1010 CFU/g after three months preservation at 4 ?C.