Influence of lactate dehydrogenase gene knockout on anaerobic production of succinic acid by Corynebacterium glutamicum

The conversion conditions of Corynebacterium glutamicum ATCC13032Δldh under anaerobic condition were investigated. The optimal carbonates were bicarbonate,and the rate of sugar consumption and succinic acid production were influenced by bicarbonate concentration. The effects of lactate dehydrogenase deletion in Corynebacterium glutamicum under anaerobic metabolism were investigated by the metabolic flux analysis and the metabolic flux to the succinic acid synthesis pathway increased by 214. 3%,while the metabolic flux to lactic acid synthesis pathway became zero. Succinic acid concentration reached 41. 2 g / L within 36 h and the yield of succinic acid was 45. 0%.