Detection of 14-3-3 Protein in 150 Samples of Cerebrospinal Fluid from CJD and Other Disorders in CNS

Aim:To evaluate clinical significance of 14-3-3 protein detection in diagnosis of Creutzfeldt- Jacob disease(CJD)and other disorders of central nervous system(CNS).Methods:150 cerebrospinal fluid (CSF)samples were detected with Western blot.26 samples of them were from cases with probable CJD (n=6)and possible CJD(n=23),clinically.Results:In all samples,19 samples showed a positive test.In 5 of 6 cases with probable CJD and 6 of 23 possible CJD were positive.Positive test was found in 1 of 6 patients with non-CJD dementia and in 3 of 7 patients with herpes simplex virus encephalitis.Positive results were also found in other disorders in CNS,including undefinite virus encephalitis(1),tuberculous meningitis and encephalitis (1),cryptococcosis(1)and cerebral infarction(l).Conclusion:Detection of 14-3-3 protein in CSF is one of the most important tests to establish clinical diagnosis of CJD,the valuation of 14-3-3 protein in diagnosis of CJD is associated with the choice of the case.