Association between intercellular adhesion molecule-1 gene polymorphism and coronary heart disease

Objective:To observe the relationship between intercellular adhesion molecule-1(ICAM-1) gene 469K/E and 241G/R polymorphism and coronary heart disease(CHD). Method:The ICAM-1 polymorphism of 103 patients with CHD and 197 normal controls were genotyped by allele-specific oligonucleotide polymerase chain reaction techniques. The plasma concentration of lipids and ICAM-1 of the subjects were measured by enzymatic method, immunonephelometry and ELISA. Result:Genotype distribution was in accordance with Hardy-Weinberg equilibrium.Allele frequencies of R was 0.030 and 0.039 in CHD and control group, respectively, whereas the distribute frequency is too small to establish the relationship between the site of ICAM-1 241G/R gene polymorphism and CHD. The genotype frequencies of 469KK, KE, EE were 36.9%, 43.7%, 19.4% and 51.8%, 31.7% , 16.5% in CHD patients and controls, respectively. Allele frequencies of K, E were 58.7%, 41.3% and 61.5% , 38.5% in CHD patients and controls, respectively (P0.05). The plasma TG and ICAM level of E carriers were distinctively higher than those of E-noncarriers (P0.05).Conclusion:The ICAM-1 469E allele is associatied with the high level of plasma TG and ICAM-1 and 469K/E polymorphism has a relationship with CHD, suggesting that E allele may be a genetic risk factor of CHD.