2005•PubMedRequires access

[c-myc gene silencing in K562 cells with RNA interference].

Feng Yue, Wenli Ma, Yanbin Song, Rong Shi, Zhang Bao, Wenling Zheng

Abstract

OBJECTIVE: To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells. METHODS: siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting. RESULTS: Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate. CONCLUSION: RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.

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Available abstract

Key concepts: K562 cells, RNA interference, Small interfering RNA, Transfection, Molecular biology, Lipofectamine, Gene silencing, Gene expression