2007•Tianjin yiyaoRequires access
Effect of siRNA on Expression of c-kit in K562 Cells
Tianxiang Pang
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Abstract
Objective:To study the effect of short interference RNA(siRNA)on the expression of c-kit in K562 cells.Methods:Plasmid pSilencer-kit expressing c-kit siRNA was constructed.The recombinant plasmid was transfected into K562 cells by lipofectamine.The cellular c-kit expression was detected by RT-PCR.To analyze the tumorigenic ability,the tumor size in nude rats,which had been injected with K562 and transfected K562 cells,was measured.Results:The level of c-kit mRNA could be significantly reduced by siRNA.When transfected with pSilencer-kit,the expressions of c-kit mRNA were significantly inhibited compared with that in control.Conclusion:The vector which express c-kit siRNA was successfully constructed.The RNA interference can effectively inhibit the expression of target gene c-kit,and can be potentially useful in gene therapy of c-kit related cancers.