Study on apoptosis induced by anthracene-9-carboxylic acid in HL-60 cells

Objective To study the effects of 9-AC on apoptosis in HL-60 cells and possible intracellular factors involved in the process.Methods HL-60 cells were treated with 9-AC to induce cell apotosis.Three international accepted criteria for monitoring apoptosis were adopted,including transmission electron microscopy,agarose gel DNA electrophoresis,and fluorescence-activated cell sorter(FACS).Results 9-AC could induce apoptosis of HL-60 cells,with its concentration most optimal at 5×10 -4?mol/L.Forty-eight hours treatment of HL-60 cells with 9-AC at above concentration induced remarkable apoptotic phenomena as typified as:Ultrastructural changes formed the so-called apoptotic bodies,typical DNA ladder,symbolizing internucleosomal fragmentation of DNA were observed by using agarose gel electrophoresis of isolated DNA from HL-60 cells.FACS analysis revealed markedly apoptotic peak-subdiploid peak.Conclusion 9-AC could induce apoptosis of HL-60 cells.