Studies on the Explant Disinfection in the Root Tissue Culture of Hevea brasiliensis

[Objective] The aim was to look for an effective way of decreasing explant contamination in the root tissue culture of Hevea brasiliensis.[Method]The root tissue culture of H.brasiliensis was carired out taking news roots after treatment for 30 d as explants,and explants was disinfected by using 1 g/L carbendazim,0.1% HgCl2 and Yipeilong with different concentration.[Result] Explants were treated by 1 g/L carbendazim before conventional disinfection and disinfected by 75% ethyl for 30 s and 0.1% HgCl2 for 6 min,successively,finally explants were inoculated in solid callus induction medium with 0.1% Yipeilong and cultured for 30 d when the contamination rate of explants decreased to 44.59% and its survival rate was up to 25.60%.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H.brasiliensis.