Expression of CD31、MMP2、MMP9、Cathepsin D on vasculogenic mimicry in the melanoma transplant tumor

Purpose To study the effect of CD31、MMP2、MMP9、Cathepsin D on vasculogenic mimicry in the melanoma transplant tumor. Methods The mouse melanoma transplant tumor model was made with B16 melanoma cell suspending liquid. When the tumor appears, choosing one mouse each day randomly according to random number chart and kill it. To get 114 cases tumor specimens totally; The tumor specimens normally HE stain ,immunohistochemistry stain and CD31-PAS double stain. Counting the positive cell around the vasculogenic mimicry and far away from vasculogenic mimicry per 100 tumor cell; Counting positive cell around the endothelial-depended vessel and far away from the endothelial-depended vessel per 100 tumor cell. Results The vasculogenic mimicry and endothelial-depended vesselco-exist in the tumor tissue at the early phase of melanoma;From day 9 to day 12,this structure was replaced by the endothelial-depended vessel. The number of CD31、MMP2 positive cell around vasculogenic mimicry was more than that of far away vasculogenic mimicry. The ratio of CD31 positive cell around that of around endothelial-depended vessel .There were correlation between vasculogenic mimicry density and the number of CD31、MMP2 positive cells around vasculogenic mimicry. The density of endothelial vessel had correlation with the number of CD31 positive cell around endothelial-depended vessel. Conclusions The vasculogenic mimicry is one sort of temporary blood-providing pattern. CD31、Cathepsin D 、MMP2、MMP9 all take part in the development of vasculogenic mimicry and CD31、MMP2 play key role in this procession.