Study of relationship between VEGFmRNA expression and vasculogenic mimicry of tumor

Objective:To explore the relationship between VEGF expression and vasculogenic mimicry of tumor in vitro.Methods:We observed the ability to form vasculogenic mimicry in several cell lines follow as LO2 cell,HepG2 cell,SMMC-7721 cell and A549 cell in three-dimensional cultures.We detected the expression of VEGF in the level of mRNA by polymerase chain reaction(PCR) in those cell lines.Results:HepG2 cell and A549 cell had the ability to form vasculogenic mimicry in three-dimensional cell cultures,while LO2 cell and SMMC-7721 cell hadn't the ability.The rates of VEGF165/GAPDH were 0.212±0.011,0.208±0.013,0.117±0.009 and 0.214±0.012 respectively,and the rate of VEGF121/GAPDH were 0.186±0.018,0.192±0.014,0.050±0.010,0.196±0.017 in LO2 cell,HepG2 cell,SMMC-7721 cell and A549 cell,respectively.The expression of VEGF gene in mRNA level in HepG2 cell,LO2 cell and A549 cell were significantly higher than that in SMMC-7721 cell(P0.05).Conclusion:The cell lines which expressed landy in VEGF don't have the ability to form vasculogenic mimicry and which expressed highly cann't form vasculogenic mimicry at all.VEGF has impact on the formation vasculgenic mimicry.Only the cells which express highly in VEGF have the potential to form vasculogenic mimicry.But it don't play an unique role in vasculogenic mimicry.