Screening of Pathogenicity Islands Genes of Porphyromonas Gingivalis with Reversing Microarray

Obiective: To investigate PG0836,PG0838 and PG0839 gene of Porphyromonas gingivalis(P.gingivalis)in subgingival plaque of the chronic periodontitis patients and periodontally healthy subjects,and to find out the relationship between detection rates of PG0836,PG0838,PG0839 and periodontal clinical parameters.Methods: The subjects were 41 Chinese CP patients and 76 periodontally healthy individuals.Clinical parameters were measured and recorded by a single examiner,including bleeding on probing(BOP),clinical attachment loss(CAL),probing depth(PD) and tooth movement(TM).Subgingival plaque samples were taken by sterile subgingival curette.The three genes,which were present in P.gingivalis W83 but absent from P.gingivalis ATCC 33277 were labeled with Cy5 and used as probes in DNA microarray hybridization to analyze DNA of disease region and normal region P.gingivalis.Results: The detection rates of PG0836,PG0838,PG0839 were different for P.gingivalis in disease region and normal region(P0.05).Conclusions: The genes of PG0836、PG0838、PG0839 were associated with pathogenicity of P.gingivalis.