2009•Shandong yiyaoRequires access
Effect of Trichostatin A on cell proliferation of breast cancer cell line MDA-MB-231
Yunchao Huang
Open publisher page 0 citations
Abstract
Objective To investigate the effect of Trichostatin A(TSA)on the cell proliferation of breast cancer cell line.Methods The breast cancer cell line MDA-MB-231 was cultured and treated with TSA in different concentrations.The cell growth was detected by MTT assay 24,48,72 and 96 hours later,the proportion of S-phase cell and expression of Cyclin A2,Cyclin D1 were measured by the flow cytometry.Results The cell growth was inhibited in time-effect manner 24-72 hours after the treatment of TSA in concentration of 1～4 μmol/L.The expression of Cyclin A2 decreased,the expression of Cyclin D1 increased after the treatment of TSA.Conclusions TSA can inhibit the cell growth of breast cancer,Cyclin A2 and Cyclin D1 involes in the regulation of cell proliferation cycle.