Effects of constant magnetic fields of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells

AIM: To explore the effects of constant magnetic fields(CMF) of different intensities on the proliferation of human umbilical arterial vascular smooth muscle cells(VSMC). METHODS: The VSMCs of human umbilical arterial were cultured with DMEM containing 100 mL·L -1 neonatal calf serum and fibroblast growth factor(5 μg·L -1 ), epithelial growth factor (10 μg·L -1 ) for 3～10 posterities. 100 μL mixture of DMEM and VSMC(cell density 1×10 5·mL -1 ) were put into the culture plate with 96 holes and incubated for 72 h in the control group which was not under a CMF. The experimental group was first incubated for 24 h, then CMFs with 0.5,1,2,5,10,30,60 mT was put into the culture plate for 48 h. MTT and 3H-TdR incorporation methods (A-value and cpm-value) were used to determine the amount of VSMC proliferation. RESULT: The amount of VSMC proliferation was significantly lower than those of the control group under the CMFs 0.5,1,2,5 mT(P0.05 ),but was significantly higher than those of the control group under the CMFs 10,30,60 mT (P0.01 ). CONCLUSION: The CMFs of 0.5,1,2,5 mT can significantly inhibit human VSMC proliferation and CMFs 10,30,60 mT can enhance VSMC proliferation.