Effects of constant magnetic field on proliferation and intracellular free calcium of human umbilical arterial vascular smooth muscle cells induced by angiotensinII

Objective To investigate the effects of constant magnetic fields (CMF) on the proliferation and intracellular free calcium of human umbilical arterial vascular smooth muscle cells (VSMC) induced by angiotensinⅡ(AngⅡ). Methods The experimental proliferation models of cultured human umbilical arterial VSMC stimulated by AngⅡ were established. VSMC were cultured under 5 mT CMF. Proliferation of VSMC was detected by MTT and 3H-TdR, cell cycle was analyzed by flow cytometry, and intracellular free calcium concentration was detected by laser scanning confocal microscope. Results 5 mT CMF could inhibit the proliferation of VSMC stimulated by AngⅡ, hold back VSMC from static phase (G0/G) to DNA synthetic(S) and mitotic phase (G2/M), and caused a persistent decreasing of intracellular free calcium concentration of VSMC from 10 min to 50 min. Conclusion It is suggested that 5 mT CMF can significantly inhibit VSMC proliferation, decrease the level of intracellular free calcium, which may be useful in treatment of RS after PTCA.